Hello Good evening.
Now we shall learn about Cuvette path length determination which is
being used under UV-Spectrophotometric analysis.
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12.5mm outer diameter of the cuvette
Here is how to calculate the
Cuvette path length:
(Wall Thickness x 2) – Outer
Dimension = Path Length
Let’s take Type 1UV10 as
Our example.
Wall thickness: 1.25 mm
Outer Dimension: 12.5 mm
1.25 x 2 = 2.5 mm
12.5 – 2.5 = 10 mm
Many analyst forget to double the wall thickness so it’s essential
to make sure you do this. See above diagram for a top view
of a 10 mm cuvette to see how we calculate the cuvette path
very good and knowledgible information sir..thank you sir..
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