Hello Good evening.

Now we shall learn about Cuvette path length determination which is being used under UV-Spectrophotometric analysis. 

 


two side of the cuvette 
 2x 1.25     mm         

               12.5mm outer diameter of the cuvette 

 And the wall thickness

Here is how to calculate the

Cuvette path length:

(Wall Thickness x 2) – Outer

Dimension = Path Length

Let’s take Type 1UV10 as

Our  example.

Wall thickness: 1.25 mm

Outer Dimension: 12.5 mm

1.25 x 2 = 2.5 mm

12.5 – 2.5 = 10 mm

Many analyst forget to double the wall thickness so it’s essential 

 to make sure you do this. See above diagram  for a top view

of a 10 mm cuvette to see how we calculate the cuvette path

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